• Newly created Oedekerk character The Thumb from Thumb Wars is seen briefly licking a giant ice cream cone.
  • Woody Woodpecker made a cameo when Agent Tommy Clark hit the players.
  • Jimmy Neutron made a cameo appearance when Agent Jackson Robinson hit Mary's head.
  • Bart Simpson made a cameo when Agent Mickey Moore hit the ground.
  • When Agent Lois watched a Max Fleischer's Cartoon Capers VHS, Kang and Kodos appear in a dream sequence.
  • Bugs Bunny made a cameo when Agent Rob watched The Wizard of Oz VHS.
  • When Agent James watching a fictional show Wombats!, Elmer Fudd, Tweety and Sylvester made a cameo appearance.
  • Betty Boop and Little Audrey made a cameo when Agent Shirley Beck hit the trash can.
  • As the credits scrolled, the agents' completed film Mystery Manners is shown. Little Lulu and Casper walks over.